Journal: Cell death and differentiation

Article Title: Fas and Fas-mediated effects on a human salivary cell line in vitro: a model for immune-mediated exocrine damage in Sjögren's syndrome.

doi: 10.1038/sj.cdd.4400414

Figure Lengend Snippet: Figure 1 Histochemical staining of minor salivary gland sections from a SS patient (A ± C) and a healthy control (D ± E). (A) represents staining after treating the section with a rabbit polyclonal anti-Fas Ab. The *symbols mark acinar cells, the single arrows (/) infiltrating lymphocytes, and the double arrows (⇓) point to ducts. In (B) the section was reacted with a polyclonal anti-FasL Ab. (C) is a section from the same specimen stained with a control polyclonal rabbit IgG Ab to demonstrate non-specific background reactivity. (D) is a section from a healthy control gland reacted with anti-Fas Ab and (E) was reacted with anti-FasL Ab. The overall staining intensity (mean of three examiners) for these sections was (A) lymphocytes = 4, ducts = 2, acini = 4.3; (B) lymphocytes = 4.3, ducts = 2, acini = 5; (D) lymphocytes = 0.5, ducts = 0, acini = 0.6; and (E) lymphocytes = 0.6, ducts = 1, acini = 1

Article Snippet: The slides were stained using an AEC kit (HistoStain SP kit, Zymed, San Francisco, CA, USA) with the following first antibodies: (i) a murine monoclonal anti-Fas antibody (ZB-4 clone, Upstate Biotechnology Inc, Lake Placid, NY, USA); (ii) a rabbit polyclonal anti-Fas Ab (SC-715 clone, Santa Cruz Biotechnology, Santa Cruz, CA, USA); and (iii) a rabbit polyclonal anti-FasL Ab (SC-957, Santa Cruz Biotechnology).

Techniques: Staining, Control

Journal: Cell death and differentiation

Article Title: Fas and Fas-mediated effects on a human salivary cell line in vitro: a model for immune-mediated exocrine damage in Sjögren's syndrome.

doi: 10.1038/sj.cdd.4400414

Figure Lengend Snippet: Figure 2 Mean staining intensity for all sections of SS (N=ten patients) and healthy (N=seven subjects) labial minor salivary glands (A) represents staining intensity for Fas reacted with the polyclonal rabbit Ab, and (B) shows the staining intensity for FasL

Article Snippet: The slides were stained using an AEC kit (HistoStain SP kit, Zymed, San Francisco, CA, USA) with the following first antibodies: (i) a murine monoclonal anti-Fas antibody (ZB-4 clone, Upstate Biotechnology Inc, Lake Placid, NY, USA); (ii) a rabbit polyclonal anti-Fas Ab (SC-715 clone, Santa Cruz Biotechnology, Santa Cruz, CA, USA); and (iii) a rabbit polyclonal anti-FasL Ab (SC-957, Santa Cruz Biotechnology).

Techniques: Staining

Journal: PLoS ONE

Article Title: Pro-Inflammatory Action of MIF in Acute Myocardial Infarction via Activation of Peripheral Blood Mononuclear Cells

doi: 10.1371/journal.pone.0076206

Figure Lengend Snippet: A . Representative images showing a temporal changes of macrophages (CD68+ cells, purple colour) by immunohistochemical staining in mice treated with isotype control IgG or anti-MIF polyclonal antibody (MIF Ab) post MI. Bar=100 μm. B , C . Grouped data showing that neutralizing MIF by anti-MIF Ab, i.p. given immediately after MI, significantly reduced density of macrophages ( B , CD68 positive cells) and leukocytes ( C , CD45+ cells) at 24 h, but had no effect on macrophage density at 7 days. n=3 for sham-operated groups (SH) and n=6-7 for each time point of MI groups. * P<0 . 05 vs. respective SH, † P<0.05 vs. respective 24 h MI, # P <0.05. D . Representative immunoblotting images for monocyte chemoattractant protein 1 (MCP-1) and CD74 in hearts from sham-operated or MI (24 h) mice treated with isotype control IgG or MIF Ab. E , F . Quantitative analysis of MCP-1 ( E ) and CD74 ( F ) expression. n=3/per sham group, n=5/per MI group. * P<0 . 05 vs. sham, # P<0.05 . G . Effects of anti-MIF Ab and isotype control IgG (CTL) treatment on healing parameters, i.e. size of residual necrotic myocardium and collagen content in the infarct area, and infarct wall thickness at 7 days post MI. n=4-6/group. H . Representative photos of ruptured hearts that occurred at 3-4 days after MI. Arrows indicate the rupture site around the border zone. I . Treatment with the MIF antagonist, COR100140, in the first 3 days post MI significantly reduced incidence of cardiac rupture. n=25 for untreated and 15 for treated groups.

Article Snippet: To investigate the effect of anti-MIF intervention on inflammatory responses, animals were treated with a single dose of anti-MIF polyclonal antibody (Santa Cruz, sc-20121) or isotype control rabbit IgG (Santa Cruz, sc-2027), at 5 mg/kg i.p. immediately after CAO.

Techniques: Immunohistochemical staining, Staining, Control, Western Blot, Expressing